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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
22/01/2021 |
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Actualizado : |
14/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. |
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Afiliación : |
RAFAEL DELPIAZZO, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; MAILA BARCELLOS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; SOFÍA BARROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; LAURA BENTANCOR, Universidad de la República Oriental del Uruguay. Facultad de Medicina. Instituto de Higiene. Departamento de Bacteriología y Virología. Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE GIL, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; GREGORIO IRAOLA, Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana, Montevideo, Uruguay. / Universidad Mayor. Facultad de Ciencias. Centro de Biología Integrativa. Santiago de Chile, Chile.; CLAUDIA MORSELLA, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; FERNANDO PAOLICCHI, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; RUBEN PEREZ, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; ALFONSO SILVA, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA CALLEROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay. |
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Título : |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 |
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DOI : |
10.1016/j.vas.2020.100163 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy |
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Contenido : |
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
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Palabras claves : |
BOVINE GENITAL CAMPYLOBACTERIOSIS; CAMPYLOBACTER FETUS; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; QPCR. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14934/1/Veterinary-Animal-Science-2021-100163.pdf
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Marc : |
LEADER 02681naa a2200373 a 4500
001 1061678
005 2021-04-14
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vas.2020.100163$2DOI
100 1 $aDELPIAZZO, R.
245 $aAccurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020. Corresponding author: laurabet@higiene.edu.uy
520 $aCampylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
653 $aBOVINE GENITAL CAMPYLOBACTERIOSIS
653 $aCAMPYLOBACTER FETUS
653 $aMOLECULAR DIAGNOSIS
653 $aMOLECULAR DIAGNOSTICS
653 $aQPCR
700 1 $aBARCELLOS, M.
700 1 $aBARROS, S.
700 1 $aBENTANCOR, L.
700 1 $aFRAGA, M.
700 1 $aGIL, J.
700 1 $aIRAOLA, G.
700 1 $aMORSELLA, C.
700 1 $aPAOLICCHI, F.
700 1 $aPÉREZ, R.
700 1 $aRIET-CORREA, F.
700 1 $aSANGUINETTI, M.
700 1 $aSILVA, A.
700 1 $aSILVEIRA, C.S.
700 1 $aCALLEROS, L.
773 $tVeterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163
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INIA Treinta y Tres (TT) |
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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
30/05/2019 |
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Actualizado : |
30/05/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SANTA CRUZ TORRES, R.; CUSHMAN, R.A.; VIÑOLES, C. |
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Afiliación : |
RODRIGO SANTA CRUZ TORRES, Pasante Programa Nacional de Investigación Producción Carne y Lana, INIA Tacuarembó. Polo Agroforestal, UdelaR, EEBR, Cerro Largo.; R.A. CUSHMAN, USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA.; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Antral follicular count is a tool that may allow the selection of more precocious Bradford heifers at weaning. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Theriogenology, v. 119, p. 35-42, 2018. |
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DOI : |
10.1016/j.theriogenology.2018.06.010 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 14 November 2017; Received in revised form 6 May 2018; Accepted 17 June 2018; Available online 19 June 2018. |
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Contenido : |
Although antral follicle count is a repeatable parameter across life that is positively associated with fertility, its use at weaning as a tool to discard less fertile heifers has not been extensively evaluated. The hypotheses of this work are: 1) maximum antral follicle count (MAFC) is repeatable between weaning and pre breeding evaluations, allowing selection of more fertile heifers at an early age, 2) heifers with high MAFC have growth and development parameters linked to an earlier puberty and regnancy, 3) MAFC has a positive correlation with AMH concentrations, so that both could be used inter changeably. In this study, Hereford (n ¼ 42 and n ¼ 50) and Braford (n ¼ 40 and n ¼ 50) females were used in years 1 and 2; respectively, in a completely randomized experimental design. Heifers were examined for five to ten days at two different moments (post weaning and pre service), to determine MAFC. The concentrations of Anti müllerian hormone (AMH) were evaluated on the day of MAFC assessment. Growth and development parameters were evaluated post weaning and pre service. The repeatability of MAFC between post weaning and pre service evaluations was poor in three cases (Hereford Year 1 ¼0.36 and 2 ¼ 0.39 and Braford, Year 2 ¼ 0.32) but it was high for Braford in Year 2 (0.72). The AMH repeatability between post weaning and pre service evaluations was high in one case (Braford Year 2 ¼ 0.72) and moderate in the others (Year 1, Hereford ¼ 0.50 and Braford ¼ 0.52 and Year 2, Hereford¼ 0.50). In Year 2, Braford heifers with greater MAFC attained puberty at an earlier age (r2 ¼ 0.129; P ¼ 0.0196). Also, diminished
MAFC corresponded with decreased growth and development, thus less Braford heifers with low MAFC were inseminated (2/16), compared to those with medium (12/17) and high MAFC (7/17; P < 0.01). Moreover, Braford heifers with low AFC had less progesterone in the cycle post insemination but pregnancy rate was not affected. In Braford heifers in Year 2, there was a high correlation between MAFC and AMH concentrations (0.85 P < 0.001). The results of these experiments indicate that post weaning MAFC and AMH concentrations may be applied to select those Braford heifers that attain puberty at an early age, but these tools are not useful in Hereford heifers. MenosAlthough antral follicle count is a repeatable parameter across life that is positively associated with fertility, its use at weaning as a tool to discard less fertile heifers has not been extensively evaluated. The hypotheses of this work are: 1) maximum antral follicle count (MAFC) is repeatable between weaning and pre breeding evaluations, allowing selection of more fertile heifers at an early age, 2) heifers with high MAFC have growth and development parameters linked to an earlier puberty and regnancy, 3) MAFC has a positive correlation with AMH concentrations, so that both could be used inter changeably. In this study, Hereford (n ¼ 42 and n ¼ 50) and Braford (n ¼ 40 and n ¼ 50) females were used in years 1 and 2; respectively, in a completely randomized experimental design. Heifers were examined for five to ten days at two different moments (post weaning and pre service), to determine MAFC. The concentrations of Anti müllerian hormone (AMH) were evaluated on the day of MAFC assessment. Growth and development parameters were evaluated post weaning and pre service. The repeatability of MAFC between post weaning and pre service evaluations was poor in three cases (Hereford Year 1 ¼0.36 and 2 ¼ 0.39 and Braford, Year 2 ¼ 0.32) but it was high for Braford in Year 2 (0.72). The AMH repeatability between post weaning and pre service evaluations was high in one case (Braford Year 2 ¼ 0.72) and moderate in the others (Year 1, Hereford ¼ 0.50 and Braford ¼ 0.52 and Year 2, Here... Presentar Todo |
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Palabras claves : |
ANTI MÜLLERIAN HORMONE; BEEF HEIFERS; FERTILITY; PUBERTY; REPLAEMENT SELECTION. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 03134naa a2200229 a 4500
001 1059795
005 2019-05-30
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2018.06.010$2DOI
100 1 $aSANTA CRUZ TORRES, R.
245 $aAntral follicular count is a tool that may allow the selection of more precocious Bradford heifers at weaning.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 14 November 2017; Received in revised form 6 May 2018; Accepted 17 June 2018; Available online 19 June 2018.
520 $aAlthough antral follicle count is a repeatable parameter across life that is positively associated with fertility, its use at weaning as a tool to discard less fertile heifers has not been extensively evaluated. The hypotheses of this work are: 1) maximum antral follicle count (MAFC) is repeatable between weaning and pre breeding evaluations, allowing selection of more fertile heifers at an early age, 2) heifers with high MAFC have growth and development parameters linked to an earlier puberty and regnancy, 3) MAFC has a positive correlation with AMH concentrations, so that both could be used inter changeably. In this study, Hereford (n ¼ 42 and n ¼ 50) and Braford (n ¼ 40 and n ¼ 50) females were used in years 1 and 2; respectively, in a completely randomized experimental design. Heifers were examined for five to ten days at two different moments (post weaning and pre service), to determine MAFC. The concentrations of Anti müllerian hormone (AMH) were evaluated on the day of MAFC assessment. Growth and development parameters were evaluated post weaning and pre service. The repeatability of MAFC between post weaning and pre service evaluations was poor in three cases (Hereford Year 1 ¼0.36 and 2 ¼ 0.39 and Braford, Year 2 ¼ 0.32) but it was high for Braford in Year 2 (0.72). The AMH repeatability between post weaning and pre service evaluations was high in one case (Braford Year 2 ¼ 0.72) and moderate in the others (Year 1, Hereford ¼ 0.50 and Braford ¼ 0.52 and Year 2, Hereford¼ 0.50). In Year 2, Braford heifers with greater MAFC attained puberty at an earlier age (r2 ¼ 0.129; P ¼ 0.0196). Also, diminished MAFC corresponded with decreased growth and development, thus less Braford heifers with low MAFC were inseminated (2/16), compared to those with medium (12/17) and high MAFC (7/17; P < 0.01). Moreover, Braford heifers with low AFC had less progesterone in the cycle post insemination but pregnancy rate was not affected. In Braford heifers in Year 2, there was a high correlation between MAFC and AMH concentrations (0.85 P < 0.001). The results of these experiments indicate that post weaning MAFC and AMH concentrations may be applied to select those Braford heifers that attain puberty at an early age, but these tools are not useful in Hereford heifers.
653 $aANTI MÜLLERIAN HORMONE
653 $aBEEF HEIFERS
653 $aFERTILITY
653 $aPUBERTY
653 $aREPLAEMENT SELECTION
700 1 $aCUSHMAN, R.A.
700 1 $aVIÑOLES, C.
773 $tTheriogenology$gv. 119, p. 35-42, 2018.
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